Rapid detection of neurotoxic insecticides in food using disposable acetylcholinesterase-biosensors
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منابع مشابه
Development, validation, and application of an acetylcholinesterase-biosensor test for the direct detection of insecticide residues in infant food.
A highly sensitive and rapid food-screening test based on disposable screen-printed biosensors was developed, which is suitable for monitoring infant food. The exposure of infants and children to neurotoxic organophosphates and carbamates is of particular concern because of their higher susceptibility to adverse effects. The European Union has, therefore, set a very low limit for pesticides in ...
متن کاملPreparation and performance of a colorimetric biosensor using acetylcholinesterase and indoxylacetate for assay of nerve agents and drugs
Different toxic compounds can target the cholinergic nervous system. Acetylcholinesterase (AChE; EC 3.1.1.7) is one of the most crucial components of the cholinergic nervous system and thus many of the toxins interact with this enzyme. As to inhibitors, nerve agents used as chemical warfare, some insecticides, and drugs influencing the cholinergic system are common examples of AChE inhibitors. ...
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This paper describes the application of electrochemical disposable biosensors in food analysis, which have recently been developed in our laboratory. Disposable biosensors, based on acetylcholinesterase inhibition activity, were exploited for testing the presence of organophosphorus and carbamate pesticides in water, fruit, and vegetable samples. The paper further describes preliminary tests fo...
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The synaptic enzyme acetylcholinesterase (AChE), which is the target of many insecticides and potential warfare agents, is implied in Alzheimer's disease and is a good potential candidate to be used in biosensors. This promotes a strong demand for production of recombinant AChE to be used in various studies. A promising expression system is the yeast Pichia pastoris, but the expression efficien...
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